Construct: shRNA TRCN0000322569

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032227.2-642s21c1
DNA Barcode:
GCTTTAAGTTCGCCACCAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM164 (84187)
Gene Description:
transmembrane protein 164
Transcript:
RefSeq NM_032227.2 (NON-CURRENT)
Match location:
Position 642 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322569)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTAAGTTCGCCACCAAGA
Hairpin Sequence:
5'-CCGG-GCTTTAAGTTCGCCACCAAGA-CTCGAG-TCTTGGTGGCGAACTTAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTAAGTTCGCCACCAAGACTCGAGTCTTGGTGGCGAACTTAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTAAGTTCGCCACCAAGACTCGAGTCTTGGTGGCGAACTTAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000141811