Construct: shRNA TRCN0000322585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015906.3-3286s21c1
DNA Barcode:
GCAGTTGCATTGTACTTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM33 (51592)
Gene Description:
tripartite motif containing 33
Transcript:
RefSeq NM_015906.3 (NON-CURRENT)
Match location:
Position 3286 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTGCATTGTACTTTGAA
Hairpin Sequence:
5'-CCGG-GCAGTTGCATTGTACTTTGAA-CTCGAG-TTCAAAGTACAATGCAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTGCATTGTACTTTGAACTCGAGTTCAAAGTACAATGCAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTGCATTGTACTTTGAACTCGAGTTCAAAGTACAATGCAACTGC-3'

Other clones with same target sequence:

  1. TRCN0000022005