Construct: shRNA TRCN0000322603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144721.4-353s21c1
DNA Barcode:
CCTTCTATCTTTGATTCTCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THAP6 (152815)
Gene Description:
THAP domain containing 6
Transcript:
RefSeq NM_144721.4 (NON-CURRENT)
Match location:
Position 353 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTATCTTTGATTCTCCA
Hairpin Sequence:
5'-CCGG-CCTTCTATCTTTGATTCTCCA-CTCGAG-TGGAGAATCAAAGATAGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTATCTTTGATTCTCCACTCGAGTGGAGAATCAAAGATAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTATCTTTGATTCTCCACTCGAGTGGAGAATCAAAGATAGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000133807