Construct: shRNA TRCN0000322681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152416.2-1404s21c1
DNA Barcode:
TACATGCTGAGCAGCTATAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDUFAF6 (137682)
Gene Description:
NADH:ubiquinone oxidoreductase complex assembly factor 6
Transcript:
RefSeq NM_152416.2 (NON-CURRENT)
Match location:
Position 1404 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACATGCTGAGCAGCTATAAC
Hairpin Sequence:
5'-CCGG-TACATGCTGAGCAGCTATAAC-CTCGAG-GTTATAGCTGCTCAGCATGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACATGCTGAGCAGCTATAACCTCGAGGTTATAGCTGCTCAGCATGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACATGCTGAGCAGCTATAACCTCGAGGTTATAGCTGCTCAGCATGTA-3'

Other clones with same target sequence:

(none)