Construct: shRNA TRCN0000322701

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018364.3-2789s21c1
DNA Barcode:
CCTTGTCTCTTGCCATATTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSBN1 (54665)
Gene Description:
round spermatid basic protein 1
Transcript:
RefSeq NM_018364.3 (NON-CURRENT)
Match location:
Position 2789 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322701)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGTCTCTTGCCATATTTG
Hairpin Sequence:
5'-CCGG-CCTTGTCTCTTGCCATATTTG-CTCGAG-CAAATATGGCAAGAGACAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGTCTCTTGCCATATTTGCTCGAGCAAATATGGCAAGAGACAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGTCTCTTGCCATATTTGCTCGAGCAAATATGGCAAGAGACAAGG-3'

Other clones with same target sequence:

(none)