Construct: shRNA TRCN0000322754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015946.4-2433s21c1
DNA Barcode:
ACGATGCCTATGCAGTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PELO (53918)
Gene Description:
pelota mRNA surveillance and ribosome rescue factor
Transcript:
RefSeq NM_015946.4 (NON-CURRENT)
Match location:
Position 2433 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGATGCCTATGCAGTATATT
Hairpin Sequence:
5'-CCGG-ACGATGCCTATGCAGTATATT-CTCGAG-AATATACTGCATAGGCATCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGATGCCTATGCAGTATATTCTCGAGAATATACTGCATAGGCATCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGATGCCTATGCAGTATATTCTCGAGAATATACTGCATAGGCATCGT-3'

Other clones with same target sequence:

(none)