Construct: shRNA TRCN0000322795

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017704.2-1189s21c1
DNA Barcode:
TGGATCTTTGTTGGGTATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD49 (54851)
Gene Description:
ankyrin repeat domain 49
Transcript:
RefSeq NM_017704.2 (NON-CURRENT)
Match location:
Position 1189 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322795)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGATCTTTGTTGGGTATTTA
Hairpin Sequence:
5'-CCGG-TGGATCTTTGTTGGGTATTTA-CTCGAG-TAAATACCCAACAAAGATCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGATCTTTGTTGGGTATTTACTCGAGTAAATACCCAACAAAGATCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGATCTTTGTTGGGTATTTACTCGAGTAAATACCCAACAAAGATCCA-3'

Other clones with same target sequence:

(none)