Construct: shRNA TRCN0000322824

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014046.3-1108s21c1
DNA Barcode:
GCACACCTGTAGTCTCAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPS18B (28973)
Gene Description:
mitochondrial ribosomal protein S18B
Transcript:
RefSeq NM_014046.3 (NON-CURRENT)
Match location:
Position 1108 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322824)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACCTGTAGTCTCAACTA
Hairpin Sequence:
5'-CCGG-GCACACCTGTAGTCTCAACTA-CTCGAG-TAGTTGAGACTACAGGTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACCTGTAGTCTCAACTACTCGAGTAGTTGAGACTACAGGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACCTGTAGTCTCAACTACTCGAGTAGTTGAGACTACAGGTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000141771