Construct: shRNA TRCN0000322834

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015986.3-1024s21c1
DNA Barcode:
CTCCAGAGCTCCGACTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRLF3 (51379)
Gene Description:
cytokine receptor like factor 3
Transcript:
RefSeq NM_015986.3 (NON-CURRENT)
Match location:
Position 1024 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322834)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCAGAGCTCCGACTTATTT
Hairpin Sequence:
5'-CCGG-CTCCAGAGCTCCGACTTATTT-CTCGAG-AAATAAGTCGGAGCTCTGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCAGAGCTCCGACTTATTTCTCGAGAAATAAGTCGGAGCTCTGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCAGAGCTCCGACTTATTTCTCGAGAAATAAGTCGGAGCTCTGGAG-3'

Other clones with same target sequence:

(none)