Construct: shRNA TRCN0000322880

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001693.3-1461s21c1
DNA Barcode:
CCGATGTATCTAACCAGCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V1B2 (526)
Gene Description:
ATPase H+ transporting V1 subunit B2
Transcript:
RefSeq NM_001693.3 (NON-CURRENT)
Match location:
Position 1461 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322880)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGATGTATCTAACCAGCTAT
Hairpin Sequence:
5'-CCGG-CCGATGTATCTAACCAGCTAT-CTCGAG-ATAGCTGGTTAGATACATCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGATGTATCTAACCAGCTATCTCGAGATAGCTGGTTAGATACATCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGATGTATCTAACCAGCTATCTCGAGATAGCTGGTTAGATACATCGG-3'

Other clones with same target sequence:

  1. TRCN0000029546