Construct: shRNA TRCN0000323121

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014761.2-1312s21c1
DNA Barcode:
GCTGCTTTCCAGTTCTCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IST1 (9798)
Gene Description:
IST1 factor associated with ESCRT-III
Transcript:
RefSeq NM_014761.2 (NON-CURRENT)
Match location:
Position 1312 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323121)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTTTCCAGTTCTCTGTT
Hairpin Sequence:
5'-CCGG-GCTGCTTTCCAGTTCTCTGTT-CTCGAG-AACAGAGAACTGGAAAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTTTCCAGTTCTCTGTTCTCGAGAACAGAGAACTGGAAAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTTTCCAGTTCTCTGTTCTCGAGAACAGAGAACTGGAAAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000129426