Construct: shRNA TRCN0000323135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006349.2-651s21c1
DNA Barcode:
GAGACTGCCTCAGTTTGATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNHIT1 (10467)
Gene Description:
zinc finger HIT-type containing 1
Transcript:
RefSeq NM_006349.2 (NON-CURRENT)
Match location:
Position 651 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACTGCCTCAGTTTGATGA
Hairpin Sequence:
5'-CCGG-GAGACTGCCTCAGTTTGATGA-CTCGAG-TCATCAAACTGAGGCAGTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACTGCCTCAGTTTGATGACTCGAGTCATCAAACTGAGGCAGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACTGCCTCAGTTTGATGACTCGAGTCATCAAACTGAGGCAGTCTC-3'

Other clones with same target sequence:

  1. TRCN0000163169