Construct: shRNA TRCN0000323140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017883.3-1780s21c1
DNA Barcode:
CGTGCACTTCTTTGATGTGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR13 (64743)
Gene Description:
WD repeat domain 13
Transcript:
RefSeq NM_017883.3 (NON-CURRENT)
Match location:
Position 1780 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCACTTCTTTGATGTGGA
Hairpin Sequence:
5'-CCGG-CGTGCACTTCTTTGATGTGGA-CTCGAG-TCCACATCAAAGAAGTGCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCACTTCTTTGATGTGGACTCGAGTCCACATCAAAGAAGTGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCACTTCTTTGATGTGGACTCGAGTCCACATCAAAGAAGTGCACG-3'

Other clones with same target sequence:

  1. TRCN0000130974