Construct: shRNA TRCN0000323175

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016570.2-1514s21c1
DNA Barcode:
GAAGATGACGCCTTCTTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERGIC2 (51290)
Gene Description:
ERGIC and golgi 2
Transcript:
RefSeq NM_016570.2 (NON-CURRENT)
Match location:
Position 1514 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323175)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATGACGCCTTCTTATAT
Hairpin Sequence:
5'-CCGG-GAAGATGACGCCTTCTTATAT-CTCGAG-ATATAAGAAGGCGTCATCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATGACGCCTTCTTATATCTCGAGATATAAGAAGGCGTCATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATGACGCCTTCTTATATCTCGAGATATAAGAAGGCGTCATCTTC-3'

Other clones with same target sequence:

(none)