Construct: shRNA TRCN0000323244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016570.2-443s21c1
DNA Barcode:
GCAGATGGTTTAGTTTATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERGIC2 (51290)
Gene Description:
ERGIC and golgi 2
Transcript:
RefSeq NM_016570.2 (NON-CURRENT)
Match location:
Position 443 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGGTTTAGTTTATGAA
Hairpin Sequence:
5'-CCGG-GCAGATGGTTTAGTTTATGAA-CTCGAG-TTCATAAACTAAACCATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGGTTTAGTTTATGAACTCGAGTTCATAAACTAAACCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGGTTTAGTTTATGAACTCGAGTTCATAAACTAAACCATCTGC-3'

Other clones with same target sequence:

  1. TRCN0000029703