Construct: shRNA TRCN0000323265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018067.3-2370s21c1
DNA Barcode:
GACTCGGAAGTCAGAAGTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP7D1 (55700)
Gene Description:
MAP7 domain containing 1
Transcript:
RefSeq NM_018067.3 (NON-CURRENT)
Match location:
Position 2370 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCGGAAGTCAGAAGTTTC
Hairpin Sequence:
5'-CCGG-GACTCGGAAGTCAGAAGTTTC-CTCGAG-GAAACTTCTGACTTCCGAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCGGAAGTCAGAAGTTTCCTCGAGGAAACTTCTGACTTCCGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCGGAAGTCAGAAGTTTCCTCGAGGAAACTTCTGACTTCCGAGTC-3'

Other clones with same target sequence:

(none)