Construct: shRNA TRCN0000323380

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005451.3-640s21c1
DNA Barcode:
GCACCTGAAGAAATCAAGCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDLIM7 (9260)
Gene Description:
PDZ and LIM domain 7
Transcript:
RefSeq NM_005451.3 (NON-CURRENT)
Match location:
Position 640 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323380)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTGAAGAAATCAAGCCA
Hairpin Sequence:
5'-CCGG-GCACCTGAAGAAATCAAGCCA-CTCGAG-TGGCTTGATTTCTTCAGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTGAAGAAATCAAGCCACTCGAGTGGCTTGATTTCTTCAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTGAAGAAATCAAGCCACTCGAGTGGCTTGATTTCTTCAGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000166005