Construct: shRNA TRCN0000323509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025934.1-468s21c4
DNA Barcode:
GCACAATGTTTCTTGGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Riok2 (67045)
Gene Description:
RIO kinase 2 (yeast)
Transcript:
RefSeq NM_025934.1 (NON-CURRENT)
Match location:
Position 468 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAATGTTTCTTGGCTTTA
Hairpin Sequence:
5'-CCGG-GCACAATGTTTCTTGGCTTTA-CTCGAG-TAAAGCCAAGAAACATTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAATGTTTCTTGGCTTTACTCGAGTAAAGCCAAGAAACATTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAATGTTTCTTGGCTTTACTCGAGTAAAGCCAAGAAACATTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000023877