Construct: shRNA TRCN0000323722

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007404.2-863s21c1
DNA Barcode:
GCTTAGCAAACTACCTGGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adam9 (11502)
Gene Description:
a disintegrin and metallopeptidase domain 9 (meltrin gamma)
Transcript:
RefSeq NM_007404.2 (CURRENT)
Match location:
Position 863 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323722)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAGCAAACTACCTGGATA
Hairpin Sequence:
5'-CCGG-GCTTAGCAAACTACCTGGATA-CTCGAG-TATCCAGGTAGTTTGCTAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAGCAAACTACCTGGATACTCGAGTATCCAGGTAGTTTGCTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAGCAAACTACCTGGATACTCGAGTATCCAGGTAGTTTGCTAAGC-3'

Other clones with same target sequence:

  1. TRCN0000031817