Construct: shRNA TRCN0000323731

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028152.3-3441s21c1
DNA Barcode:
CCAGAGAATAAAGCCTTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mms19 (72199)
Gene Description:
MMS19 (MET18 S. cerevisiae)
Transcript:
RefSeq NM_028152.3 (CURRENT)
Match location:
Position 3441 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323731)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGAATAAAGCCTTCTTT
Hairpin Sequence:
5'-CCGG-CCAGAGAATAAAGCCTTCTTT-CTCGAG-AAAGAAGGCTTTATTCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGAATAAAGCCTTCTTTCTCGAGAAAGAAGGCTTTATTCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGAATAAAGCCTTCTTTCTCGAGAAAGAAGGCTTTATTCTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000096384