Construct: shRNA TRCN0000323749

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028803.3-1187s21c1
DNA Barcode:
CGGGTGACTATAATGAATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gbe1 (74185)
Gene Description:
glucan (1,4-alpha-), branching enzyme 1
Transcript:
RefSeq NM_028803.3 (NON-CURRENT)
Match location:
Position 1187 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323749)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGTGACTATAATGAATATT
Hairpin Sequence:
5'-CCGG-CGGGTGACTATAATGAATATT-CTCGAG-AATATTCATTATAGTCACCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGTGACTATAATGAATATTCTCGAGAATATTCATTATAGTCACCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGTGACTATAATGAATATTCTCGAGAATATTCATTATAGTCACCCG-3'

Other clones with same target sequence:

  1. TRCN0000093951