Construct: shRNA TRCN0000323966

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013626.3-3115s21c1
DNA Barcode:
GCCATGTTTATTCGATGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pam (18484)
Gene Description:
peptidylglycine alpha-amidating monooxygenase
Transcript:
RefSeq NM_013626.3 (CURRENT)
Match location:
Position 3115 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323966)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTTTATTCGATGGAAA
Hairpin Sequence:
5'-CCGG-GCCATGTTTATTCGATGGAAA-CTCGAG-TTTCCATCGAATAAACATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTTTATTCGATGGAAACTCGAGTTTCCATCGAATAAACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTTTATTCGATGGAAACTCGAGTTTCCATCGAATAAACATGGC-3'

Other clones with same target sequence:

  1. TRCN0000076340