Construct: shRNA TRCN0000324016

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133975.4-5904s21c1
DNA Barcode:
CGGGCTGTGAAGTTCTTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trip12 (14897)
Gene Description:
thyroid hormone receptor interactor 12
Transcript:
RefSeq NM_133975.4 (CURRENT)
Match location:
Position 5904 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324016)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGCTGTGAAGTTCTTGTTT
Hairpin Sequence:
5'-CCGG-CGGGCTGTGAAGTTCTTGTTT-CTCGAG-AAACAAGAACTTCACAGCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGCTGTGAAGTTCTTGTTTCTCGAGAAACAAGAACTTCACAGCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGCTGTGAAGTTCTTGTTTCTCGAGAAACAAGAACTTCACAGCCCG-3'

Other clones with same target sequence:

  1. TRCN0000039425