Construct: shRNA TRCN0000324030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018753.6-793s21c1
DNA Barcode:
CCTGAATGAAGAGTCTTACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ywhab (54401)
Gene Description:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
Transcript:
RefSeq NM_018753.6 (CURRENT)
Match location:
Position 793 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAATGAAGAGTCTTACAA
Hairpin Sequence:
5'-CCGG-CCTGAATGAAGAGTCTTACAA-CTCGAG-TTGTAAGACTCTTCATTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAATGAAGAGTCTTACAACTCGAGTTGTAAGACTCTTCATTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAATGAAGAGTCTTACAACTCGAGTTGTAAGACTCTTCATTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000076400