Construct: shRNA TRCN0000324054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026097.2-492s21c1
DNA Barcode:
GACCTGCTTGGACTGTAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rffl (67338)
Gene Description:
ring finger and FYVE like domain containing protein
Transcript:
RefSeq NM_026097.2 (NON-CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTGCTTGGACTGTAAGAA
Hairpin Sequence:
5'-CCGG-GACCTGCTTGGACTGTAAGAA-CTCGAG-TTCTTACAGTCCAAGCAGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTGCTTGGACTGTAAGAACTCGAGTTCTTACAGTCCAAGCAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTGCTTGGACTGTAAGAACTCGAGTTCTTACAGTCCAAGCAGGTC-3'

Other clones with same target sequence:

  1. TRCN0000040622