Construct: shRNA TRCN0000324219

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029447.2-1695s21c1
DNA Barcode:
GCACGATTTAGTGGAACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nln (75805)
Gene Description:
neurolysin (metallopeptidase M3 family)
Transcript:
RefSeq NM_029447.2 (CURRENT)
Match location:
Position 1695 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324219)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACGATTTAGTGGAACAAAT
Hairpin Sequence:
5'-CCGG-GCACGATTTAGTGGAACAAAT-CTCGAG-ATTTGTTCCACTAAATCGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACGATTTAGTGGAACAAATCTCGAGATTTGTTCCACTAAATCGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACGATTTAGTGGAACAAATCTCGAGATTTGTTCCACTAAATCGTGC-3'

Other clones with same target sequence:

  1. TRCN0000031156