Construct: shRNA TRCN0000324341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008396.2-1345s21c1
DNA Barcode:
CCCGTGATCTTTCCTAAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itga2 (16398)
Gene Description:
integrin alpha 2
Transcript:
RefSeq NM_008396.2 (NON-CURRENT)
Match location:
Position 1345 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTGATCTTTCCTAAACAA
Hairpin Sequence:
5'-CCGG-CCCGTGATCTTTCCTAAACAA-CTCGAG-TTGTTTAGGAAAGATCACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTGATCTTTCCTAAACAACTCGAGTTGTTTAGGAAAGATCACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTGATCTTTCCTAAACAACTCGAGTTGTTTAGGAAAGATCACGGG-3'

Other clones with same target sequence:

  1. TRCN0000065894