Construct: shRNA TRCN0000324365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013841.2-2431s21c1
DNA Barcode:
GAATGTCATGGCCTCCTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps45 (22365)
Gene Description:
vacuolar protein sorting 45
Transcript:
RefSeq NM_013841.2 (NON-CURRENT)
Match location:
Position 2431 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGTCATGGCCTCCTCTTA
Hairpin Sequence:
5'-CCGG-GAATGTCATGGCCTCCTCTTA-CTCGAG-TAAGAGGAGGCCATGACATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGTCATGGCCTCCTCTTACTCGAGTAAGAGGAGGCCATGACATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGTCATGGCCTCCTCTTACTCGAGTAAGAGGAGGCCATGACATTC-3'

Other clones with same target sequence:

  1. TRCN0000093294