Construct: shRNA TRCN0000324380

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026896.4-754s21c1
DNA Barcode:
GCCCTGCTTCACTATCAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Med27 (68975)
Gene Description:
mediator complex subunit 27
Transcript:
RefSeq NM_026896.4 (NON-CURRENT)
Match location:
Position 754 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324380)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGCTTCACTATCAGTTA
Hairpin Sequence:
5'-CCGG-GCCCTGCTTCACTATCAGTTA-CTCGAG-TAACTGATAGTGAAGCAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGCTTCACTATCAGTTACTCGAGTAACTGATAGTGAAGCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGCTTCACTATCAGTTACTCGAGTAACTGATAGTGAAGCAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000096040