Construct: shRNA TRCN0000324438
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_013841.2-1538s21c1
- DNA Barcode:
- CCGAGATTGGTAGCAATATAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Vps45 (22365)
- Gene Description:
- vacuolar protein sorting 45
- Transcript:
- RefSeq NM_013841.2 (NON-CURRENT)
- Match location:
- Position 1538 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000324438)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCGAGATTGGTAGCAATATAA
- Hairpin Sequence:
-
5'-CCGG-CCGAGATTGGTAGCAATATAA-CTCGAG-TTATATTGCTACCAATCTCGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCGAGATTGGTAGCAATATAACTCGAGTTATATTGCTACCAATCTCGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCGAGATTGGTAGCAATATAACTCGAGTTATATTGCTACCAATCTCGG-3'
Other clones with same target sequence:
-
TRCN0000093295
