Construct: shRNA TRCN0000324442

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029825.2-65s21c1
DNA Barcode:
GAAGCGGATGTTGAATTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scfd1 (76983)
Gene Description:
Sec1 family domain containing 1
Transcript:
RefSeq NM_029825.2 (NON-CURRENT)
Match location:
Position 65 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324442)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCGGATGTTGAATTTCAA
Hairpin Sequence:
5'-CCGG-GAAGCGGATGTTGAATTTCAA-CTCGAG-TTGAAATTCAACATCCGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCGGATGTTGAATTTCAACTCGAGTTGAAATTCAACATCCGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCGGATGTTGAATTTCAACTCGAGTTGAAATTCAACATCCGCTTC-3'

Other clones with same target sequence:

  1. TRCN0000093307