Construct: shRNA TRCN0000324573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025358.2-205s21c1
DNA Barcode:
CCTGGGTCGATACGTTGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufa9 (66108)
Gene Description:
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9
Transcript:
RefSeq NM_025358.2 (NON-CURRENT)
Match location:
Position 205 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGTCGATACGTTGTTAA
Hairpin Sequence:
5'-CCGG-CCTGGGTCGATACGTTGTTAA-CTCGAG-TTAACAACGTATCGACCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGTCGATACGTTGTTAACTCGAGTTAACAACGTATCGACCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGTCGATACGTTGTTAACTCGAGTTAACAACGTATCGACCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000041544