Construct: shRNA TRCN0000324657

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009035.4-1486s21c1
DNA Barcode:
CCAGTGACTTTGGTCCGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbpj (19664)
Gene Description:
recombination signal binding protein for immunoglobulin kappa J region
Transcript:
RefSeq NM_009035.4 (NON-CURRENT)
Match location:
Position 1486 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324657)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGACTTTGGTCCGAAAT
Hairpin Sequence:
5'-CCGG-CCAGTGACTTTGGTCCGAAAT-CTCGAG-ATTTCGGACCAAAGTCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGACTTTGGTCCGAAATCTCGAGATTTCGGACCAAAGTCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGACTTTGGTCCGAAATCTCGAGATTTCGGACCAAAGTCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000097286