Construct: shRNA TRCN0000324683

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146083.1-159s21c1
DNA Barcode:
CTGGTAAAGGAGAGTTAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srsf7 (225027)
Gene Description:
serine/arginine-rich splicing factor 7
Transcript:
RefSeq NM_146083.1 (NON-CURRENT)
Match location:
Position 159 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324683)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGTAAAGGAGAGTTAGAAA
Hairpin Sequence:
5'-CCGG-CTGGTAAAGGAGAGTTAGAAA-CTCGAG-TTTCTAACTCTCCTTTACCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGTAAAGGAGAGTTAGAAACTCGAGTTTCTAACTCTCCTTTACCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGTAAAGGAGAGTTAGAAACTCGAGTTTCTAACTCTCCTTTACCAG-3'

Other clones with same target sequence:

  1. TRCN0000071937