Construct: shRNA TRCN0000324723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009104.1-1125s21c1
DNA Barcode:
TCTCACTAGAAGGAAAGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rrm2 (20135)
Gene Description:
ribonucleotide reductase M2
Transcript:
RefSeq NM_009104.1 (NON-CURRENT)
Match location:
Position 1125 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTCACTAGAAGGAAAGACAA
Hairpin Sequence:
5'-CCGG-TCTCACTAGAAGGAAAGACAA-CTCGAG-TTGTCTTTCCTTCTAGTGAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTCACTAGAAGGAAAGACAACTCGAGTTGTCTTTCCTTCTAGTGAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTCACTAGAAGGAAAGACAACTCGAGTTGTCTTTCCTTCTAGTGAGA-3'

Other clones with same target sequence:

  1. TRCN0000042402