Construct: shRNA TRCN0000324761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146120.3-2050s21c1
DNA Barcode:
CGCTTTGTGATCGAAGAGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gsn (227753)
Gene Description:
gelsolin
Transcript:
RefSeq NM_146120.3 (NON-CURRENT)
Match location:
Position 2050 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTTGTGATCGAAGAGGTT
Hairpin Sequence:
5'-CCGG-CGCTTTGTGATCGAAGAGGTT-CTCGAG-AACCTCTTCGATCACAAAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTTGTGATCGAAGAGGTTCTCGAGAACCTCTTCGATCACAAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTTGTGATCGAAGAGGTTCTCGAGAACCTCTTCGATCACAAAGCG-3'

Other clones with same target sequence:

  1. TRCN0000071928