Construct: shRNA TRCN0000324826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023126.2-1490s21c1
DNA Barcode:
CCATGAAATGAATCTGTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab8a (17274)
Gene Description:
RAB8A, member RAS oncogene family
Transcript:
RefSeq NM_023126.2 (CURRENT)
Match location:
Position 1490 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGAAATGAATCTGTCTTT
Hairpin Sequence:
5'-CCGG-CCATGAAATGAATCTGTCTTT-CTCGAG-AAAGACAGATTCATTTCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGAAATGAATCTGTCTTTCTCGAGAAAGACAGATTCATTTCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGAAATGAATCTGTCTTTCTCGAGAAAGACAGATTCATTTCATGG-3'

Other clones with same target sequence:

  1. TRCN0000100420