Construct: shRNA TRCN0000324856

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133216.2-1553s21c1
DNA Barcode:
TGTGGGATTCTGGTCTGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xpnpep1 (170750)
Gene Description:
X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Transcript:
RefSeq NM_133216.2 (NON-CURRENT)
Match location:
Position 1553 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324856)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGGGATTCTGGTCTGGATT
Hairpin Sequence:
5'-CCGG-TGTGGGATTCTGGTCTGGATT-CTCGAG-AATCCAGACCAGAATCCCACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGGGATTCTGGTCTGGATTCTCGAGAATCCAGACCAGAATCCCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGGGATTCTGGTCTGGATTCTCGAGAATCCAGACCAGAATCCCACA-3'

Other clones with same target sequence:

  1. TRCN0000031938