Construct: shRNA TRCN0000324980

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019718.2-326s21c1
DNA Barcode:
GCCGACAGAAAGAGATTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arl3 (56350)
Gene Description:
ADP-ribosylation factor-like 3
Transcript:
RefSeq NM_019718.2 (CURRENT)
Match location:
Position 326 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324980)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGACAGAAAGAGATTTGAA
Hairpin Sequence:
5'-CCGG-GCCGACAGAAAGAGATTTGAA-CTCGAG-TTCAAATCTCTTTCTGTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGACAGAAAGAGATTTGAACTCGAGTTCAAATCTCTTTCTGTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGACAGAAAGAGATTTGAACTCGAGTTCAAATCTCTTTCTGTCGGC-3'

Other clones with same target sequence:

  1. TRCN0000100926