Construct: shRNA TRCN0000325052

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019401.2-402s21c1
DNA Barcode:
CGGATGCCAGAGAATTCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nmi (64685)
Gene Description:
N-myc (and STAT) interactor
Transcript:
RefSeq NM_019401.2 (CURRENT)
Match location:
Position 402 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325052)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATGCCAGAGAATTCCAAA
Hairpin Sequence:
5'-CCGG-CGGATGCCAGAGAATTCCAAA-CTCGAG-TTTGGAATTCTCTGGCATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATGCCAGAGAATTCCAAACTCGAGTTTGGAATTCTCTGGCATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATGCCAGAGAATTCCAAACTCGAGTTTGGAATTCTCTGGCATCCG-3'

Other clones with same target sequence:

  1. TRCN0000100978