Construct: shRNA TRCN0000325189

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008247.2-450s21c1
DNA Barcode:
CCTCGATGTGATTTGCGTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plpp1 (19012)
Gene Description:
phospholipid phosphatase 1
Transcript:
RefSeq NM_008247.2 (NON-CURRENT)
Match location:
Position 450 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325189)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGATGTGATTTGCGTGTT
Hairpin Sequence:
5'-CCGG-CCTCGATGTGATTTGCGTGTT-CTCGAG-AACACGCAAATCACATCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGATGTGATTTGCGTGTTCTCGAGAACACGCAAATCACATCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGATGTGATTTGCGTGTTCTCGAGAACACGCAAATCACATCGAGG-3'

Other clones with same target sequence:

  1. TRCN0000080878