Construct: shRNA TRCN0000325211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030675.2-2049s21c1
DNA Barcode:
CGTACCTATTACTAAACTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krit1 (79264)
Gene Description:
KRIT1, ankyrin repeat containing
Transcript:
RefSeq NM_030675.2 (NON-CURRENT)
Match location:
Position 2049 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTACCTATTACTAAACTGAA
Hairpin Sequence:
5'-CCGG-CGTACCTATTACTAAACTGAA-CTCGAG-TTCAGTTTAGTAATAGGTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTACCTATTACTAAACTGAACTCGAGTTCAGTTTAGTAATAGGTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTACCTATTACTAAACTGAACTCGAGTTCAGTTTAGTAATAGGTACG-3'

Other clones with same target sequence:

  1. TRCN0000105936