Construct: shRNA TRCN0000325381
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_007805.4-425s21c1
- DNA Barcode:
- CTTAGTCTTTGTTCTGTACTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Cyb561 (13056)
- Gene Description:
- cytochrome b-561
- Transcript:
- RefSeq NM_007805.4 (CURRENT)
- Match location:
- Position 425 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000325381)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTTAGTCTTTGTTCTGTACTT
- Hairpin Sequence:
-
5'-CCGG-CTTAGTCTTTGTTCTGTACTT-CTCGAG-AAGTACAGAACAAAGACTAAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTTAGTCTTTGTTCTGTACTTCTCGAGAAGTACAGAACAAAGACTAAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTTAGTCTTTGTTCTGTACTTCTCGAGAAGTACAGAACAAAGACTAAG-3'
Other clones with same target sequence:
-
TRCN0000127046
