Construct: shRNA TRCN0000325422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133835.1-529s21c1
DNA Barcode:
GCTCCAGACAAAGATGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ubac1 (98766)
Gene Description:
ubiquitin associated domain containing 1
Transcript:
RefSeq NM_133835.1 (NON-CURRENT)
Match location:
Position 529 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCAGACAAAGATGCTATT
Hairpin Sequence:
5'-CCGG-GCTCCAGACAAAGATGCTATT-CTCGAG-AATAGCATCTTTGTCTGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCAGACAAAGATGCTATTCTCGAGAATAGCATCTTTGTCTGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCAGACAAAGATGCTATTCTCGAGAATAGCATCTTTGTCTGGAGC-3'

Other clones with same target sequence:

  1. TRCN0000113262