Construct: shRNA TRCN0000325438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010716.2-3003s21c1
DNA Barcode:
CCAGACTTCAAACGTCTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lig3 (16882)
Gene Description:
ligase III, DNA, ATP-dependent
Transcript:
RefSeq NM_010716.2 (NON-CURRENT)
Match location:
Position 3003 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACTTCAAACGTCTCAAA
Hairpin Sequence:
5'-CCGG-CCAGACTTCAAACGTCTCAAA-CTCGAG-TTTGAGACGTTTGAAGTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACTTCAAACGTCTCAAACTCGAGTTTGAGACGTTTGAAGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACTTCAAACGTCTCAAACTCGAGTTTGAGACGTTTGAAGTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000070978