Construct: shRNA TRCN0000325445

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028946.3-1085s21c1
DNA Barcode:
CCATCAGGAATGTTCCGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc9b1 (74446)
Gene Description:
solute carrier family 9, subfamily B (NHA1, cation proton antiporter 1), member 1
Transcript:
RefSeq NM_028946.3 (CURRENT)
Match location:
Position 1085 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325445)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCAGGAATGTTCCGATTA
Hairpin Sequence:
5'-CCGG-CCATCAGGAATGTTCCGATTA-CTCGAG-TAATCGGAACATTCCTGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCAGGAATGTTCCGATTACTCGAGTAATCGGAACATTCCTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCAGGAATGTTCCGATTACTCGAGTAATCGGAACATTCCTGATGG-3'

Other clones with same target sequence:

  1. TRCN0000127081