Construct: shRNA TRCN0000325454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178920.3-352s21c1
DNA Barcode:
GTCTTCTACTTCGGAGCGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mal2 (105853)
Gene Description:
mal, T cell differentiation protein 2
Transcript:
RefSeq NM_178920.3 (NON-CURRENT)
Match location:
Position 352 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTCTACTTCGGAGCGTTT
Hairpin Sequence:
5'-CCGG-GTCTTCTACTTCGGAGCGTTT-CTCGAG-AAACGCTCCGAAGTAGAAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTCTACTTCGGAGCGTTTCTCGAGAAACGCTCCGAAGTAGAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTCTACTTCGGAGCGTTTCTCGAGAAACGCTCCGAAGTAGAAGAC-3'

Other clones with same target sequence:

  1. TRCN0000100651