Construct: shRNA TRCN0000325465
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_033521.3-1027s21c1
- DNA Barcode:
- GCATGAATTATCCTGTGGTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Laptm4b (114128)
- Gene Description:
- lysosomal-associated protein transmembrane 4B
- Transcript:
- RefSeq NM_033521.3 (CURRENT)
- Match location:
- Position 1027 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000325465)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCATGAATTATCCTGTGGTTT
- Hairpin Sequence:
-
5'-CCGG-GCATGAATTATCCTGTGGTTT-CTCGAG-AAACCACAGGATAATTCATGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCATGAATTATCCTGTGGTTTCTCGAGAAACCACAGGATAATTCATGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCATGAATTATCCTGTGGTTTCTCGAGAAACCACAGGATAATTCATGC-3'
Other clones with same target sequence:
-
TRCN0000110795
