Construct: shRNA TRCN0000325504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_212446.1-317s21c1
DNA Barcode:
CCAGCTAAGTGGAACAGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psmf1 (228769)
Gene Description:
proteasome (prosome, macropain) inhibitor subunit 1
Transcript:
RefSeq NM_212446.1 (NON-CURRENT)
Match location:
Position 317 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTAAGTGGAACAGCAAT
Hairpin Sequence:
5'-CCGG-CCAGCTAAGTGGAACAGCAAT-CTCGAG-ATTGCTGTTCCACTTAGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTAAGTGGAACAGCAATCTCGAGATTGCTGTTCCACTTAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTAAGTGGAACAGCAATCTCGAGATTGCTGTTCCACTTAGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000066615