Construct: shRNA TRCN0000325540

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020276.3-1659s21c1
DNA Barcode:
GCAGATGATTGAGACCTACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nsmf (56876)
Gene Description:
NMDA receptor synaptonuclear signaling and neuronal migration factor
Transcript:
RefSeq NM_020276.3 (CURRENT)
Match location:
Position 1659 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325540)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGATTGAGACCTACTT
Hairpin Sequence:
5'-CCGG-GCAGATGATTGAGACCTACTT-CTCGAG-AAGTAGGTCTCAATCATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGATTGAGACCTACTTCTCGAGAAGTAGGTCTCAATCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGATTGAGACCTACTTCTCGAGAAGTAGGTCTCAATCATCTGC-3'

Other clones with same target sequence:

  1. TRCN0000089491